Chitosan is insoluble in water and most organic solvents, which seriously limits both its application scope and applicable fields. These results concluded that both of OCh and ChNP have a positive effect on the productive performance of laying hens.Ĭhitosan is a product of the deacetylation of chitin, which is widely found in nature. Total bacterial count, including Lactobacillus count in the intestine was significantly increased (P < 0.05) in the T1 and T3 groups compared to the other groups. Immunoglobulin Y was significantly increased (P<0.05) in the T1 group followed by decreasing order in the T3 group. Malondialdehyde concentration was significantly decreased in the T1 and T3 groups (P<0.05) compared to the Co group.

However, saturated fatty acids (ΣSFA) were significantly decreased in the T1 and T3 groups compared to the other groups (P<0.05). Unsaturated fatty acids (ΣUSFA) were significantly increased in the T1 and T3 compared to the other groups (P<0.05). Albumen height and Haugh unit were significantly increased in the T1 and T3 groups compared to the control group (P<0.05). Egg mass and feed intake were significantly decreased (P<0.05) for all treatments compared to the control group. The egg laying rate, average egg weight, and feed conversion ratio of hens were not affected significantly by the experimental groups. The experimental period was 12 weeks (23-34 weeks old). The control group (Co) were fed the basal diet without the supplement, while birds in the treatment groups T 1, T 2, and T 3 were fed the basal diet supplemented with 200 mg OCh/kg diet, 50 mg ChNP/kg diet, and 200 mg ChNP /kg diet, respectively. The control (Co) group, T1 group, and T2 group were fed 0.0 mg/kg diet, 150 mg/kg diet and 300 mg/kg diet, respectively. A total of 96 hens of Bábolna TETRA-SL at 23 weeks of age were randomly assigned into 4 experimental groups with three replicates each. The present study evaluated the effects different forms of chitosan (ordinary chitosan ' OCh' and chitosan nanoparticles ' ChNP ') as a dietary supplementation on egg production, egg quality, yolk egg composition, blood serum traits, and intestinal bacteria.

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